Медицинская литература. Новинки

 

 

 

 

 

 

Ассоциация между полиморфизмом гена гемоксигеназы-1 (HMOX1) и хроническим панкреатитом

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Цель. Выявление ассоциации полиморфизма rs2071746 гена HMOX1 с хроническим панкреатитом (ХП) в азербайджанской популяции. Материал и методы. Изучили 70 пациентов с различными видами ХП с 2014 по 2019 г. и 90 практически здоровых добровольцев - 55 употребляющих алкоголь как минимум 3-4 раза в неделю в средней дозе >80 г/л в день и 35 не употребляющих алкоголь. Образцы венозной крови, взятые у пациентов и у лиц контрольной группы, после проведения экстракции ДНК подвергли полимеразной цепной реакции - полиморфизму длин рестрикционных фрагментов (ПЦР-ПДРФ). Результаты. Аллель А в гомозиготной форме (генотип А/А) чаще наблюдали у больных XП (42,9%), чем в контрольной группе (16,6%). Таким образом, аллель А в гомозиготной форме (генотип А/А) можно считать ассоциированным с развитием ХП (OR = 3,75; 95% CI 1,81-7,77; p = 0,000). Также была обнаружена статистическая корреляция аллеля А полиморфизма -495 A>T (rs2071746) с повышенным риском заболевания (OR = 1,73; 95% CI 1,09-2,74; p = 0,02). Аллель А в гетерозиготной форме (генотип А/Т; OR = 0,27; 95% CI 0,13-0,56) и аллель Т полиморфизма (OR = 0,53; 95% CI 0,32-0,89) -495 A>T (rs2071746) HMOX1 уменьшали риск развития алкогольного ХП (p = 0,000 и p = 0,017). Заключение. Обнаружение генетической ассоциации с различными видами ХП станет толчком к изучению HMOX1, индукции в лекарства или применению гемина в клинических условиях.

Ключевые слова:
поджелудочная железа, хронический панкреатит, генетические факторы, полиморфизм, HMOX1, pancreas, chronic pancreatitis, genetic factors, polymorphism, HMOX1

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Association between heme oxygenase-1 (HMOXT) gene polymorphism and chronic pancreatitis

Aim: To investigate the association of the heme oxygenase-1 (HMOX1) gene polymorphism rs2071746 and chronic pancreatitis in the Azerbaijani population. Methods: From a period of 2014-2019, 70 patients with different types of chronic pancreatitis and 90 apparently healthy volunteers were observed. Of them, 55 subjects were consuming alcohol at least 3-4 times a week, with an average daily dose of >80 g/L, and 35 subjects were not consuming alcohol. The venous blood samples were collected from the control subjects and patients with pancreatitis; DNA was extracted and analyzed by polymerase chain reaction-restriction fragment length polymorphism. Results: The A allele in the homozygous form (A/A genotype) was found to be more common in patients with chronic pancreatitis (42.9%) than in the control group (16.6%). Thus, the A allele in the homozygous form (A/A genotype) may be associated with the development of chronic pancreatitis (odd ratio [OR] = 3.75; 95% confidence interval [CI] 1.81-7.77; p = 0.000). Furthermore, a statistical correlation between the A allele of the 495 A>T polymorphism (rs2071746) and an increased risk of the disease was observed. (p = 0.02, OR = 1.73; 95% CI 1.09-2.74). The heterozygous form of the A allele (A/T genotype; OR = 0.27; 95% CI = 0.13-0.56) and the T allele of the HMOX1 -495 A>T (rs2071746) polymorphism (OR = 0.53; 95% CI 0.32-0.89) reduced the risk of developing chronic alcoholic pancreatitis (p = 0.000 and p = 0.017, respectively). Conclusion: The identification of genetic association of the various types of chronic pancreatitis will encourage further research on HMOX1, drug development, or the use of hemin in clinical practice.

Keywords:
поджелудочная железа, хронический панкреатит, генетические факторы, полиморфизм, HMOX1, pancreas, chronic pancreatitis, genetic factors, polymorphism, HMOX1